Lorenzo Vannozzi

Ethylene vinyl acetate (EVA) is a copolymer comprehending the semi-crystalline polyethylene and amorphous vinyl acetate phases, which potentially allow the fabrication of tunable materials. This paper aims at describing the fabrication and characterization of nanocomposite thin films made of polyethylene vinyl acetate, at different polymer concentration and vinyl acetate content, doped with piezoelectric nanomaterials, namely zinc oxide and barium titanate. These membranes are prepared by solvent casting, achieving a thickness in the order of 100–200 µm. The nanocomposites are characterized in terms of morphological, mechanical, and chemical properties. Analysis of the nanocomposites shows the nanofillers to be homogeneously dispersed in EVA matrix at different vinyl acetate content. Their influence is also noted in the mechanical behavior of thin films, which elastic modulus ranged from about 2 to 25 MPa, while keeping an elongation break from 600% to 1500% and tensile strength from 2 up to 13 MPa. At the same time, doped nanocomposite materials increase their crystallinity degree than the bare ones. The radiopacity provided by the addition of the dopant agents is proven. Finally, the direct piezoelectricity of nanocomposites membranes is demonstrated, showing higher voltage outputs (up to 2.5 V) for stiffer doped matrices. These results show the potentialities provided by the addition of piezoelectric nanomaterials towards mechanical reinforcement of EVA-based matrices while introducing radiopaque properties and responsiveness to mechanical stimuli.

In this paper, a novel nanofilm type is proposed based on a blend of poly(ethylene glycol)-block-poly(ε-caprolactone) methyl ether (PEG-b-PCL) and poly(l-lactic acid), doped with zinc oxide nanoparticles (ZnO NPs) at different concentrations (0.1, 1, and 10 mg/mL). All nanofilm types were featured by a thickness value of ∼500 nm. Increasing ZnO NP concentrations implied larger roughness values (∼22 nm for the bare nanofilm and ∼67 nm for the films with 10 mg/mL of NPs), larger piezoelectricity (average d33 coefficient for the film up to ∼1.98 pm/V), and elastic modulus: the nanofilms doped with 1 and 10 mg/mL of NPs were much stiffer than the nondoped controls and nanofilms doped with 0.1 mg/mL of NPs. The ZnO NP content was also directly proportional to the material melting point and crystallinity and inversely proportional to the material degradation rate, thus highlighting the stabilization role of ZnO particles. In vitro tests were carried out with cells of the musculoskeletal apparatus (fibroblasts, osteoblasts, chondrocytes, and myoblasts). All cell types showed good adhesion and viability on all substrate formulations. Interestingly, a higher content of ZnO NPs in the matrix demonstrated higher bioactivity, boosting the metabolic activity of fibroblasts, myoblasts, and chondrocytes and enhancing the osteogenic and myogenic differentiation. These findings demonstrated the potential of these nanocomposite matrices for regenerative medicine applications, such as tissue engineering.